谷氨酰胺干预断奶仔猪肠道黏膜受损中免疫能力的作用

doi:10.11843/j.issn.0366-6964.2017.04.019

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (4): 752-760.doi: 10.11843/j.issn.0366-6964.2017.04.019

谷氨酰胺干预断奶仔猪肠道黏膜受损中免疫能力的作用

黄牛¹, 李欢¹, 何流琴^2,3, 田军权^2,3, 崔志杰⁴, 姚康^1,2*

1. 湖南农业大学, 动物科学技术学院, 长沙 410128;
2. 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 湖南省畜禽健康养殖工程技术中心, 农业部中南动物营养与饲料科学观测实验站, 长沙 410125;
3. 中国科学院大学, 北京 100049;
4. 湘潭大学, 湘潭 411105

收稿日期:2016-10-14 出版日期:2017-04-23 发布日期:2017-04-23
通讯作者: 姚康，研究员，博士，主要从事动物生态营养与分子生物学研究，E-mail：yaokang@isa.ac.cn
作者简介:黄牛（1992-），男，湖北十堰人，硕士生，主要从事动物营养与饲料科学研究，E-mail：452990460@qq.com
基金资助:
国家973课题（2013CB127301）；中国科学院百人计划项目；国家自然科学基金面上项目（31472107）

The Effects of Glutamine on the Immunity in the Intestinal Mucosa of Weaned Piglets with Lipopolysaccharide Challenge

HUANG Niu¹, LI Huan¹, HE Liu-qin^2,3, TIAN Jun-quan^2,3, CUI Zhi-jie⁴, YAO Kang^1,2*

1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Center of Healthy Livestock, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central of Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China;
4. Xiangtan University, Xiangtan 411105, China

Received:2016-10-14 Online:2017-04-23 Published:2017-04-23

摘要/Abstract

摘要：

本试验旨在研究日粮中添加谷氨酰胺（Gln）对大肠杆菌型脂多糖（LPS）诱导肠道黏膜损伤后断奶仔猪免疫性能的影响。选取28日龄健康断奶仔猪24头，随机分为3组，每组8个重复。其中，空白对照组和LPS组饲喂常规基础日粮，Gln组饲喂添加1%谷氨酰胺的基础日粮。试验期30 d，于试验期第22、25、28、30天对LPS组和Gln组腹腔注射LPS（按体重以100 μg·kg^-1给予），空白对照组腹腔注射等体积的无菌生理盐水。第30天对仔猪进行前腔静脉采血并屠宰采取所需样品，检测血清中免疫球蛋白、相关炎症因子浓度及各肠段中这些炎症因子基因的表达丰度。与空白对照组相比，LPS组和Gln组血清IgM水平显著提高40.00%和62.86%（P<0.05）；LPS组血清IL-8含量显著提高7.11%（P<0.05），而Gln组则无明显变化。不论在LPS攻毒前还是攻毒后，日粮添加Gln均可显著提高仔猪血清IL-17含量（P<0.05）。与空白对照组相比，LPS组和Gln组十二指肠中IL-8 mRNA表达水平显著降低了35.06%和44.16%（P<0.05）。对于IL-12β mRNA的表达水平，在空肠和回肠中Gln组中的表达水平较LPS组高，但在十二指肠中有所降低（P>0.05）。在十二指肠与空肠中，IL-17α mRNA的表达丰度在三组间存在差异（P<0.05），特别是在十二指肠和回肠中，Gln组的表达丰度达到最高；LPS组和Gln组空肠中IL-17α mRNA相对表达量显著降低58.16%和56.07%（P<0.05）。结果提示，日粮中添加1%的谷氨酰胺能够在一定程度上改善LPS诱导肠道损伤后断奶仔猪的免疫力，减轻应激反应造成的机体损伤。

Abstract:

This study was conducted to evaluate the effects of dietary glutamine (Gln) supplementation on the immunity in the lipopolysaccharide (LPS)-challenged weaned piglets. A total of twenty-four healthy piglets were weaned at 28 days, and randomly assigned into three treatment groups (8 pigs per treatment): (1)control group (basal diet); (2) LPS group (basal diet); (3)Gln group (basal diet+1.0% glutamine).The experiment lasted for 30 days. On day 22, 25, 28 and 30 of the trial, piglets in the LPS and Gln groups were administered intraperitoneally with LPS (100 μg·kg^-1 body weight), whereas pigs in the control group were injected intraperitoneally with the same volume of sterile saline. After the whole feeding period, blood samples were taken from jugular vein, then pigs were bled by exsanguination to collect samples, which were used to determine the immune parameters. The results showed that, compared with the control group, the concentration of serum IgM in the Gln and LPS groups remarkably increased by 40.00% and 62.86% (P<0.05), respectively. After LPS challenge, the concentration of serum interleukin-8 (IL-8) in the LPS group was significantly decreased by 7.11% (P<0.05), but there was no difference between the Gln group and control group (P>0.05). No matter with LPS challenge or not, Gln supplementation was remarkably increased serum interleukin-17 (IL-17) content(P<0.05).Compared with the control group, in the duodenum, the mRNA expression of IL-8 in LPS group and Gln group was remarkably decreased by 35.06% (P<0.05) and 44.16% (P<0.05). The mRNA expression of IL-12β in the jejunum and ileum of Gln group were higher than that in the LPS group (P>0.05). In the duodenum and jejunum, there were differences in the mRNA expression of IL-17α among the three groups (P<0.05), and the expression of IL-17α in the Gln group was the greatest in the duodenum and ileum; in the jejunum, the mRNA expression of IL-17α in LPS group and Gln group was remarkably decreased by 58.16% (P<0.05) and 56.07% (P<0.05). Collectively, dietary supplemented with 1% Gln could improve the immunity of weaned piglets challenged with LPS to some degree, thereby alleviating immunological stress.

中图分类号:

S852.4

黄牛, 李欢, 何流琴, 田军权, 崔志杰, 姚康. 谷氨酰胺干预断奶仔猪肠道黏膜受损中免疫能力的作用[J]. 畜牧兽医学报, 2017, 48(4): 752-760.

HUANG Niu, LI Huan, HE Liu-qin, TIAN Jun-quan, CUI Zhi-jie, YAO Kang. The Effects of Glutamine on the Immunity in the Intestinal Mucosa of Weaned Piglets with Lipopolysaccharide Challenge[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 752-760.

参考文献

[1] RHOADS J M, WU G Y. Glutamine, arginine, and leucine signaling in the intestine[J]. Amino Acids, 2009, 37(1):111-122.
[2] CURI R, LAGRANHA C J, DOI S Q, et al. Molecular mechanisms of glutamine action[J]. J Cell Physiol, 2005, 204(2):392-401.
[3] WU G, KNABE D A. Free and protein-bound amino acids in sow's colostrum and milk[J]. J Nutr, 1994, 124(3):415-424.
[4] 肖英平, 洪奇华, 刘秀婷, 等. 谷氨酰胺对断奶仔猪生长性能、营养物质表观消化率、空肠碱性磷酸酶活性及与肠道健康相关因子基因表达的影响[J]. 动物营养学报, 2012, 24(8):1438-1446.
XIAO Y P, HONG Q H, LIU X T, et al. Effects of glutamine on growth performance, apparent digestibility of nutrients, jejunal alkaline phosphatase activity and expression of genes related to intestinal health in weaner piglets[J]. Chinese Journal of Animal Nutrition, 2012, 24(8):1438-1446. (in Chinese)
[5] 褚永康, 胡静, 朱亚骏, 等. 谷氨酰胺的作用机制及其在仔猪生产中的应用[J]. 中国饲料, 2013(17):22-25.
CHU Y K, HU J, ZHU Y J, et al. Function mechanism of glutamine and its application in piglets production[J]. China Feed, 2013(17):22-25. (in Chinese)
[6] WANG J J, CHEN L X, LI P, et al. Gene expression is altered in piglet small intestine by weaning and dietary glutamine supplementation[J]. J Nutr, 2008, 138(6):1025-1032.
[7] 张军民, 王连递, 高振川, 等. 日粮添加谷氨酰胺对早期断奶仔猪抗氧化能力的影响[J]. 畜牧兽医学报, 2002, 33(2):105-109.
ZHANG J M, WANG L D, GAO Z C, et al. Effect of dietary glutamine supplementation on antioxidant capacity of tissue in the Early-weaned piglets[J]. Acta Veterinaria et Zootechnica Sinica, 2002, 33(2):105-109. (in Chinese)
[8] 孟腾飞, 韩愈杰, 王敬, 等. 猪血清免疫球蛋白IgG的分离纯化及抗大肠杆菌作用[J]. 饲料工业, 2015(7):34-37.
MENG T F, HAN Y J, WANG J, et al. Separation and purification of IgG from pig serum and the determination of concentration[J]. Feed Industry, 2015(7):34-37. (in Chinese)
[9] MÖLLER R, HANSEN G H, DANIELSEN E M. IgG trafficking in the adult pig small intestine:one-or bidirectional transfer across the enterocyte brush border[J]. Histochem Cell Biol, 2016, doi:10.1007/s00418-016-1492-x.
[10] 陈静, 刘显军, 张飞, 等. 谷氨酰胺对免疫应激仔猪免疫器官指数的影响[J]. 中国兽医杂志, 2010, 46(9):3-5.
CHEN J, LIU X J, ZHANG F, et al. Effects of dietary glutamine on immune organ in inflammatory-stressed piglets[J]. Chinese Journal of Veterinary Medicine, 2010, 46(9):3-5. (in Chinese)
[11] 梁艳, 顾以韧, 曾凯, 等. 不同猪种泌乳期母乳中IgM的动态变化[J]. 养猪, 2016(3):71-72.
LIANG Y, GU Y R, ZENG K, et al. Dynamic changes of the IgM in different pig breeds during lactation[J]. Swine Production, 2016(3):71-72. (in Chinese)
[12] CHEN C C, CHANG H M. Effect of thermal protectants on the stability of bovine milk immunoglobulin G[J]. J Agric Food Chem, 1998, 46(9):3570-3576.
[13] YU I T, WU J F, YANG P C, et al. Roles of glutamine and nucleotides in combination in growth, immune responses and FMD antibody titres of weaned pigs[J]. Anim Sci, 2002, 75(3):379-385.
[14] ZHONG X, LI W, HUANG X X, et al. Effects of glutamine supplementation on the immune status in weaning piglets with intrauterine growth retardation[J]. Arch Anim Nutr, 2012, 66(5):347-356.
[15] ARUMUGAM T, FUJⅡ T, HAOJIE H, et al. Interleukin-8(IL-8) expression is a surrogate marker for effects of gemcitabine on pancreatic ductal adenocarcinoma (PDAC) cells[J]. Pancreatology, 2013, 13(2):e5.
[16] ROSTAMI K, NEJAD M R, ASADZADEH H, et al. PTU-199 proinflammatory cytokine (IL-8) in microscopic enteritis[J]. Gut, 2013, 62(S1):A130.
[17] VAN WANROOIJ R L, ZWIERS A, KRAAL G, et al. Genetic variations in interleukin-12 related genes in immune-mediated diseases[J]. J Autoimmun, 2012, 39(4):359-368.
[18] 马樱, 金伯泉. IL-12家族新成员及其在免疫应答中的重要调节作用[J]. 细胞与分子免疫学杂志, 2008, 24(11):1129-1132.
MA Y, JIN B Q. New members of the IL-12 family and its important regulatory role in immune response[J]. Chinese Journal of Cellular and Molecular Immunology, 2008, 24(11):1129-1132. (in Chinese)
[19] KOMIYAMA Y, NAKAE S, MATSUKI T, et al. IL-17 plays an important role in the development of experimental autoimmune encephalomyelitis[J]. J Immunol, 2006, 177(1):566-573.
[20] MENG X Y, ZHOU C H, MA J, et al. Expression of interleukin-17 and its clinical significance in gastric cancer patients[J]. Med Oncol, 2012, 29(5):3024-3208.
[21] WIJTTEN P J A, VAN DER MEULEN J, VERSTEGEN M W. Intestinal barrier function and absorption in pigs after weaning:a review[J]. Br J Nutr, 2011, 105(7):967-981.
[22] 戴四发,李有志,李如兰,等.外源性谷氨酰胺对肉仔鸡空肠形态结构的影响[J].畜牧兽医学报,2005,36(1):100-104.
DAI S F, LI Y Z, LI R L, et al. Influence of glutamine supplementation on jejunum morphological structure in broiler chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2005,36(1):100-104.(in Chinese)
[23] 聂新志, 蒋宗勇, 林映才, 等. 精氨酸和谷氨酰胺对猪小肠上皮细胞增殖的影响及机理探讨[J]. 中国农学通报, 2012, 28(2):1-5.
NIE X Z, JIANG Z Y, LIN Y C, et al. Effect of arginine and glutamine addition on the proliferation of porcine intestinal epithelial cell and its mechanism[J]. Chinese Agricultural Science Bulletin, 2012, 28(2):1-5. (in Chinese)
[24] KANAGAWA N, GAO J Q, MOTOMURA Y, et al. Antitumor mechanism of intratumoral injection with IL-12-expressing adenoviral vector against IL-12-unresponsive tumor[J]. Biochem Biophys Res Commun, 2008, 372(4):821-825.
[25] SONG X Y, HE X, LI X X, et al. The roles and functional mechanisms of interleukin-17 family cytokines in mucosal immunity[J]. Cell Mol Immunol, 2016, 13(4):418-431.

谷氨酰胺干预断奶仔猪肠道黏膜受损中免疫能力的作用

The Effects of Glutamine on the Immunity in the Intestinal Mucosa of Weaned Piglets with Lipopolysaccharide Challenge

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